Analysis Note

ControlCerebellar tissue sections or homogenates

Application

Anti-Metabotropic Glutamate Receptor 1α Antibody, pain, C-terminus is an antibody against Metabotropic Glutamate Receptor 1α for use in IH(P), IH & WB.

Western blot: 0.25-1.0 μg/mL. Detects a band migrating at approximately 140 kDa. The use of membrane preparations and protease inhibitors is highly recommended.

Immunohistochemistry: AB1551 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-2.0 μg/mL.

The antibody has not been tested for use in immunoprecipitation. However, antibodies to the carboxy termini of other glutamate receptors are very effective in immunoprecipitation.

Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: C-terminus

Carboxy terminal peptide of rat mGluR1alpha conjugated to KLH with glutaraldehyde (PNVTYASVILRDYKQSSSTL).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1595

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.

ImmunoAffinity Purified

Specificity

Recognizes metabotropic glutamate receptor 1a (mGluR1a). On western blots of rat brain microsomes, the antibody labels a single band migrating at 140 kDa, corresponding to mGluR1alpha (Testa, 1998). The antibody recognizes a 140 kDa band in extracts from transfected cells expressing mGluR1alpha but not in extracts from transfected cells expressing mGluR5 (Marino, 2001).

Storage and Stability

Maintain lyophilized material at 2–8°C or -20°C. Reconstitute to 500 µL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.

Target description

140 kDa